Table 1.
Presence of the Trp53R270H mutant allele mediates alterations in the expression levels of validated transcriptional targets of wildtype p53. The expression of 16 genes that are known transcriptional targets of wildtype p53 was impacted by the presence of the Trp53R270H mutant allele. It is noteworthy that allele dosage effects were observed for some genes; Bax, Ccng1, Cdkn1a, Gdf15, Mdm2. Data shown are fragments per kilobase of exon model per million mapped reads (FPKM).
| Gene | p Value (ANOVA) |
Trp53+/+ (Mouse #1) |
Trp53+/+ (Mouse #2) |
Trp53+/R270H (Mouse #1) |
Trp53+/R270H (Mouse #2) |
Trp53R270H/R270H (Mouse #1) |
Trp53R270H/R270H (Mouse #2) |
|---|---|---|---|---|---|---|---|
| Acta1 | 0.03226382 | 459.72382 | 723.69403 | 1295.8693 | 1305.2902 | 1532.4598 | 1335.5698 |
| Bax | 0.00234206 | 92.46413 | 90.38328 | 67.5547 | 58.88641 | 26.7726 | 21.443594 |
| Bbc3 | 0.00355776 | 22.755802 | 18.623598 | 13.3205 | 11.639098 | 6.8682103 | 6.6584005 |
| Cav1 | 0.00357872 | 82.86131 | 84.6536 | 83.98589 | 89.3961 | 113.60498 | 110.93498 |
| Ccng1 | 0.00381259 | 216.86192 | 178.55894 | 80.588196 | 99.719315 | 50.149597 | 54.22179 |
| Cdkn1a | 2.50 × 10−5 | 108.96001 | 100.64 | 40.4964 | 41.430794 | 6.70245 | 6.1654506 |
| Ddit4 | 0.04823983 | 57.755894 | 79.54468 | 45.9636 | 41.706512 | 35.910603 | 27.632603 |
| Gdf15 | 0.01252794 | 30.834797 | 61.762997 | 22.0756 | 10.823901 | 2.20235 | 2.4798 |
| Hsp90ab1 | 0.03196126 | 669.3211 | 686.84717 | 753.2858 | 651.8237 | 433.094 | 504.98203 |
| Mdm2 | 0.00440876 | 53.018593 | 45.430508 | 26.794598 | 29.479399 | 18.8087 | 21.066896 |
| Nos3 | 0.04553094 | 1.25112 | 1.57297 | 2.18129 | 2.02219 | 2.01661 | 2.03199 |
| Ppm1j | 0.03864105 | 1.14598 | 2.0899 | 4.44729 | 4.88169 | 4.1659994 | 3.9569898 |
| Prkab1 | 0.03862554 | 65.31939 | 59.280407 | 60.98009 | 58.167007 | 46.9706 | 50.6644 |
| Ptk2b | 0.04042444 | 15.3253975 | 15.081601 | 14.640602 | 12.964701 | 11.9638 | 11.0372 |
| Tap1 | 0.04544611 | 22.922 | 17.592104 | 14.591501 | 12.070802 | 11.3092985 | 10.970899 |
| Tnfrsf10b | 0.00248734 | 6.25948 | 5.06814 | 1.88841 | 2.3068597 | 1.18625 | 1.04909 |
heterozygous (Trp53+/R270H) and/or or homozygous mice (Trp53R270H/R270H). A dose-dependent effect was observed for Mgmt. The expression of 4 genes which are associated with prostate cancer incidence and/or disease progression (Cdkn1a, Kras, Raf1, and Cav1) were impacted by the presence of the Trp53R270H allele. A gene dosage effect was observed for 1 of these, Cdkn1a. Data shown are fragments per kilobase of exon model per million mapped reads (FPKM).