Table 1.
Biochemical, physical and biomechanical characterization of decellularized and devitalized Wharton’s Jelly (n = 5).
| DC-WJ | DV-WJ | p | |
|---|---|---|---|
| DNA content (ng/mg of the dry tissue) | Below the limit detection | 664.12 ± 210.75 | 0.007 |
| Collagen (mg/mg of dry tissue) | 0.88 ± 0.09 | 0.89 ± 0.06 | 0.8 |
| Sulfated GAGs (μg/mg of dry tissue) | 3.14 ± 1.4 | 1.6 ± 0.6 | 0.2 |
| Non sulfated GAGs (μg/mg of dry tissue) | 15.08 ± 3.4 | 15.11 ± 1.9 | 0.9 |
| Released VEGF (pg/mL/mg of dry tissue) | 126 ± 17 | 37 ± 17 | 0.01 |
| Released HGF (pg/mL/mg of dry tissue) | 451 ± 148 | 296 ± 54 | 0.16 |
| Released TGF-β (pg/mL/mg of dry tissue) | 445 ± 25 | 436 ± 24 | 0.67 |
| Porosity range (μm) | 10 to 184 | 0.1 to 184 | - |
| Total porosity (%) | ~79 | - | |
| Swelling ratio (g/g) | 14.0 ± 6.7 | 18.5 ± 9.2 | 0.2 |
| Linear elastic modulus (dry condition) (MPa) | 12.69 ± 3.87 | 12.57 ± 3.08 | 0.77 |
| Linear elastic modulus (wet condition) (MPa) | 0.64 ± 0.22 | 0.61 ± 0.11 | 0.89 |
(VEGF: vascular endothelial growth factor, HGF: hepatic growth factor, TGF-β: Transforming growth factor beta). p values from Wilcoxon-Mann-Whitney test.