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. 2022 Jan 29;10(2):319. doi: 10.3390/biomedicines10020319

Figure 1.

Figure 1

Effect of metformin on human isolated monocytes’ polarization into M1 and M2 macrophages. Human monocytes were isolated from blood of healthy donors and incubated in the absence or presence of metformin (2 mM) and GM-CSF (50 ng/mL) and LPS (100 ng/mL) for 7 days and analyzed by flow cytometry using fluorescent anti-CD14, anti-CD16, and anti-CD80 antibodies (A). Human monocytes were isolated from blood of healthy donors and incubated in the absence or presence of metformin (2 mM) and M-CSF (50 ng/mL) and IL-4 (20 ng/mL) for 7 days and analyzed by flow cytometry using fluorescent anti-CD14, anti-CD16, and anti-CD163 and anti-CD200R antibodies (B). Cell sorting and profiling was completed using a FACS Canto II cytometer and DIVA software for analysis. Data are mean of fluorescence (n = 3). * p value < 0.05.