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Evaluation of the cytotoxic effects of metformin on monocytes and M2 polarized macrophages. Human monocytes (A–C) and M2 macrophages (D) were incubated in the absence or the presence of metformin (0.5, 1, and 2 mM) for different indicated time periods. Cytotoxicity was analyzed after the addition of cytoX reagent and measurement of absorbance at 450 nm. Data are mean of absorbance ± SD (n = 3).
