Figure 4.

Effect of metformin on the expression of the NADPH oxidase components. M2 macrophages were incubated in the absence or the presence of 2 mM metformin then stimulated by LPS (4 µg/mL) for 1 and 3 h. The expression of gp91^phox, p22^phox, p47^phox, and p67^phox was evaluated compared to actin by SDS-PAGE and Western blot using specific antibodies (A). The gp91^phox (B,C), p67^phox (D,E), p47^phox (F,G), p22^phox (H,I) and actin bands from three experiments were quantified and expressed as mean ± SD.