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. 2022 Jan 29;10(2):319. doi: 10.3390/biomedicines10020319

Figure 5.

Figure 5

Effect of metformin on the phosphorylation of Ser345 of p47phox and its upstream MAP kinases, p38MAPK, and ERK1/2 in M2 macrophages. M2 macrophages were incubated in the absence or the presence of 2 mM metformin, then stimulated by LPS (4 µg/mL) for 1 and 3 h. The phosphorylation of Ser345-p47phox, p38MAPK, and ERK1/2 was evaluated compared to actin by SDS-PAGE and Western blot using specific antibodies (A). The pSer345-p47phox (B,C), p38MAPK (D,E), and ERK1/2 (F,G) and actin bands from three experiments were quantified and expressed as mean ± SD.