Figure 7.

Effect of AMPK activation in human isolated monocytes and AMPK depletion in murine whole blood and bone marrow cells on ROS production. Human monocytes were incubated in the absence or the presence of AICAR (1 mM), stimulated by fMLF (100 nM), and ROS production was measured by luminol-amplified chemiluminescence (A). ROS production of whole blood cells (B) and bone marrow cells (C) isolated from of wild-type mice and AMPKα1-/- mice increased in the presence of fMLF (100 nM). A stronger response to fMLF was observed with AMPKα1-/- versus wild-type cells. The induced luminol-amplified chemiluminescence was measured using the luminometer for 15 min. Data are mean of chemiluminescence (n = 3). * p value < 0.05 and ** p value < 0.01.