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. 2022 Feb 16;12(2):316. doi: 10.3390/biom12020316

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterization of BMSCs and BMSC-EVs. (A) After flow cytometry BMSCs identification via physical parameters and singlets selection, (B) analysis of general MSC (CD44, CD73, CD90 and CD105), adult MSC (CD271) and hemato-endothelial (CD31, CD34 and CD45) markers, confirms BMSCs identity. In panel (B), representative plots of FITC (CD34/90), PE (CD73/271), PC5 (CD31/105) and PC7 (CD44/45) channels for events gated under “Singlets” in panel (A) are shown. (C) Representative NTA analysis of BMSC-EVs. (D) Flow cytometry of FITC-labeled nanoparticles assuring calibration of flow cytometer and comparison with CFSE-labeled BMSC-EVs. (E) Presence of MSC-markers CD44, CD73 and CD90 and EV-markers CD9, CD63 and CD81 on CFSE-labeled BMSC-EVs. In panel (E), representative plots of APC channel for all tested antibodies for events gated under FITC⁺ gate of EVs + CFSE in panel (D) are shown.