Table 1.
Forty-seven modulated proteins in PDAC secretome of knock-down p53 Paca3 cells, as compared to wild-type p53 cells identified by SWATH-MS technology (p < 0.05). Fold change (FC) represents the ratio between CM protein abundance of p53 knock-down (KD) and p53 wild-type PaCa3 cells.
| Uniprot ID | Uniprot Accession Name |
Protein Name | Gene Name | FC (KD/wt p53) | p-Value |
|---|---|---|---|---|---|
| O00410 | IPO5_HUMAN | Importin-5 | IPO5 | 4.7 | 4.31 × 10−4 |
| O75874 | IDHC_HUMAN | Isocitrate dehydrogenase | IDH1 | 3.0 | 3.56 × 10−4 |
| P01008 | ANT3_HUMAN | Antithrombin-III | SERPINC1 | 1.6 | 2.66 × 10−4 |
| P02786 | TFR1_HUMAN | Transferrin receptor protein 1 | TFRC | 3.8 | 8.59 × 10−4 |
| P04792 | HSPB1_HUMAN | Heat shock protein beta-1 | HSPB1 | 1.8 | 1.72 × 10−2 |
| P04908 | H2A1_HUMAN | Histone H2A type 1-B/E | HIST1 | 2.1 | 2.39 × 10−3 |
| P07478 | TRY2_HUMAN | Trypsin-2 | PRSS2 | 1.3 | 4.27 × 10−2 |
| P07858 | CATB_HUMAN | Cathepsin B | CTSB | 2.6 | 3.33 × 10−3 |
| P14136 | K2C8_HUMAN | Glial fibrillary acidic protein | GFAP | 5.5 | 1.49 × 10−2 |
| P14555 | PA2GA_HUMAN | Phospholipase A2 | PLA2G2A | 2.4 | 1.86 × 10−4 |
| P19338 | NUCL_HUMAN | Nucleolin | NCL | 1.4 | 9.66 × 10−4 |
| P22692 | IBP4_HUMAN | Insulin-like growth factor-binding protein 4 | IGFBP4 | 1.9 | 9.48 × 10−3 |
| P25398 | RS12_HUMAN | 40S ribosomal protein S12 | RPS12 | 1.6 | 2.92 × 10−2 |
| P37837 | TALDO_HUMAN | Transaldolase | TALDO1 | 1.4 | 4.35 × 10−3 |
| P40925 | MDHC_HUMAN | Malate dehydrogenase | MDH1 | 1.8 | 9.26 × 10−3 |
| P53396 | ACLY_HUMAN | ATP-citrate synthase | ACLY | 2.9 | 6.18 × 10−4 |
| P54652 | HSP7C_HUMAN | Heat shock-related 70 kDa protein 2 | HSPA2 | 1.7 | 6.79 × 10−3 |
| P62158 | CALM_HUMAN | Calmodulin | CALM1 | 1.5 | 1.31 × 10−2 |
| P80188 | NGAL_HUMAN | Neutrophil gelatinase-associated lipocalin | LCN2 | 2.0 | 7.20 × 10−5 |
| Q01105 | SET_HUMAN | Protein SET | SET | 1.6 | 1.55 × 10−2 |
| Q04828 | AK1C1_HUMAN | Aldo-keto reductase family 1 member C1 | AKR1C1 | 1.5 | 8.20 × 10−3 |
| Q15582 | BGH3_HUMAN | Transforming growth factor-beta-induced protein ig-h3 | TGFBI | 1.4 | 5.93 × 10−3 |
| Q32P51 | RA1L2_HUMAN | Heterogeneous nuclear ribonucleoprotein A1-like 2 | HNRNPA1L2 | 1.3 | 7.76 × 10−3 |
| Q5H9F3 | BCORL_HUMAN | BCL-6 corepressor-like protein 1 | BCORL1 | 24.3 | 5.74 × 10−5 |
| Q6UWE0 | LRSM1_HUMAN | E3 ubiquitin-protein ligase LRSAM1 | LRSAM1 | 2.2 | 6.48 × 10−3 |
| Q86TI0 | TBCD1_HUMAN | TBC1 domain family member 1 | TBC1D1 | 2.5 | 8.13 × 10−5 |
| Q92598 | HS105_HUMAN | Heat shock protein 105 kDa | HSPH1 | 1.9 | 1.65 × 10−2 |
| Q96HU8 | DIRA2_HUMAN | GTP-binding protein Di-Ras2 | DIRAS2 | 36.4 | 4.02 × 10−4 |
| Q96QV6 | H2A1A_HUMAN | Histone H2A type 1-A | HIST1H2AA | 1.3 | 1.29 × 10−3 |
| A8MST6 | CEP78_HUMAN | Centrosomal protein of 78 kDa | CEP78 | 23.7 | 2.99 × 10−3 |
| B2RPK0 | HGB1A_HUMAN | Putative high mobility group protein B1-like 1 | HMGB1P1 | 0.6 | 8.43 × 10−3 |
| O14556 | G3P_HUMAN | Glyceraldehyde-3-phosphate dehydrogenase | GAPDHS | 0.5 | 2.66 × 10−2 |
| P01034 | CYTC_HUMAN | Cystatin-C | CST3 | 0.6 | 4.58 × 10−3 |
| P02538 | K2C6B_HUMAN | Keratin, type II cytoskeletal 6A | KRT6A | 0.5 | 1.53 × 10−3 |
| P02768 | ALBU_HUMAN | Serum albumin | ALB | 0.6 | 4.06 × 10−4 |
| P07737 | PROF1_HUMAN | Profilin-1 | PFN1 | 0.5 | 9.28 × 10−6 |
| P08238 | HS90B_HUMAN | Heat shock protein HSP 90-beta | HSP90AB1 | 0.6 | 1.14 × 10−2 |
| P14324 | FPPS_HUMAN | Farnesyl pyrophosphate synthase | FDPS | 0.3 | 9.93 × 10−4 |
| P23284 | PPIB_HUMAN | Peptidyl-prolyl cis-trans isomerase B | PPIB | 0.7 | 1.40 × 10−2 |
| P26447 | S10A4_HUMAN | Protein S100-A4 | S100A4 | 0.3 | 1.72 × 10−4 |
| P37802 | TAGL2_HUMAN | Transgelin-2 | TAGLN2 | 0.3 | 2.18 × 10−3 |
| P60709 | ACTB_HUMAN | Actin, cytoplasmic 1 | ACTB | 0.6 | 5.29 × 10−5 |
| Q04760 | LGUL_HUMAN | Lactoylglutathione lyase | GLO1 | 0.5 | 5.79 × 10−4 |
| Q13885 | TBB5_HUMAN | Tubulin beta-3 chain | TUBB3 | 0.3 | 2.70 × 10−3 |
| Q58FG1 | HS90A_HUMAN | Putative heat shock protein HSP 90-alpha A4 | HSP90AA4P | 0.3 | 2.16 × 10−3 |
| Q92688 | AN32B_HUMAN | Acidic leucine-rich nuclear phosphoprotein 32 family member B | ANP32B | 0.4 | 5.73 × 10−3 |
| Q9Y536 | PPIA_HUMAN | Peptidyl-prolyl cis-trans isomerase A-like 4A | PPIAL4A | 0.4 | 3.52 × 10−3 |