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. 2022 Jan 29;10(2):320. doi: 10.3390/biomedicines10020320

Figure 4.

Figure 4

Effect of nanosized zingerone on cell invasion of three human OSCC cell lines. Cells were treated with various doses of zingerone and/or zingerone NPs and then subjected to Boyden chamber assays. Following incubation for an adequate time, the cell invasion of three human OSCC cell lines, (A) Ca9-22 cells, (B) Ca1-27 cells, and (C) SAS cells, was imaged and recorded using inverted microscopy. Bar: 100 µm. The invasive cells of (D) Ca9-22 cells, (E) Ca1-27 cells, and (F) SAS cells were further counted and quantified. Bars represented as mean ± SEM for n = 5 membranes in three separate experiments (* p < 0.05; ** p < 0.01).