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Medicarpin induces the NRF2 transcriptional level in Hela cells. Cells were treated with 50 μM of medicarpin for 6 h in the condition of transfection with an NRF2-promoter luciferase construct. NRF2-luciferase activity was measured using a Dual-Luciferase Reporter Assay kit (Promega) according to the manufacturer’s instructions. Experiments were performed in quintuplicate. *** p < 0.0001.
