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. 2022 Feb 16;11(2):404. doi: 10.3390/antiox11020404

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Melting curves observed for qPCR analysis of the relative expression of PAL, POD, SOD, WRKYa, PAL1, DEF-1, CAT-2, WRKY40, HSFC1, LOX2, and NPR1 genes normalized to UBI3 after different treatments: mock, RS (R. solanacearum), KA9 + RS (Bacillus subtilis + R. solanacearum), PDS1 + RS (Pseudomonas fluorescens + R. solanacearum), KA9 + PDS1 + RS (Bacillus subtilis + Pseudomonas fluoresecens + R. solanacearum), and BABA (chemical control). Melting curve (A) and amplification plot (B) observed for qPCR analysis of relative expression of normalized Ubi3 gene in chili. (C) Genes yielding a single melting curve. (D) Genes shown multiple peaks (marked by red arrows) were excluded from the analysis.