Figure 5.
Effects of 3,5,7-trimethoxyflavone (6) on MAPK phosphorylation in normal human dermal fibroblasts (NHDFs) stimulated by TNF-α. (A) Bands of protein expressions. (B) Bar graphs of the relative expression. NHDFs were seeded at a density of 3 × 105 cells/well in 6-well plates and incubated for 24 h, and then the medium was replaced with a serum-free medium to create starvation conditions. After 24 h, cells were treated with 50 and 100 μM of 6 for 1 h, and the cells were exposed to 20 ng/mL TNF-α for 15 min. Relative comparison of expression levels of p-ERK, ERK, p-JNK, JNK, p-p38, p38, and GAPDH proteins were determined with Western blotting. Results of each protein expression was presented as percent of the vehicle control. The results were obtained through three replicate experiments, and the graphs are represented as mean ± SEM. # p < 0.05 vs. vehicle control. * p < 0.05 vs. TNF-α stimulated control.