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. 2022 Feb 10;12:819580. doi: 10.3389/fonc.2022.819580

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Copyright © 2022 Johnson, Uppalapati, Pascual, Estrada, Averitte, Leyva and Hull

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CFH in Routine Mohs and Advanced cSCC Samples by IHC. cSCC removed from routine clinic patients by Mohs surgery [fixed after cryosectioning; (A)] and an array of advanced cSCC [formalin fixed; (B)] were labeled with mouse anti-CFH (OX-24) and an AP-conjugated secondary antibody with permanent red stain. Mayer’s Hematoxylin was used as a counterstain (nuclei). The degree of CFH staining in the ANT and cSCC samples was semi-quantitatively determined by using a 0-3+ scale, with 0 indicating no staining, 1+ indicating <10% staining, 2+ indicating 10-50% staining, and 3+ indicating >50% staining. ANT samples [(A, B), top panels] were scored as either 0 or 1+, the Mohs cSCC samples [(A), bottom panel] were scored as 2+, and the advanced cSCC samples [(B), bottom panel] were scored as 3+. The boxes within the 40x and 100x images delineate the tissue location shown in the 100x and 400x images, respectively.