Figure 6.

FOXP3 in Routine Mohs and Advanced cSCC Samples by IHC. cSCC removed from routine clinic patients by Mohs surgery [fixed after cryosectioning; (A)] and an array of advanced cSCC [formalin fixed; (B)] were labeled with a rabbit monoclonal anti-FOXP3 (Cell Marque) and stained with an HRP-conjugated secondary antibody and DAB stain (for Array samples) and with an AP-conjugated secondary antibody and permanent red stain (for ALBR samples). Mayer’s Hematoxylin was used as a counterstain (nuclei). The degree of FOXP3 staining in the ANT and cSCC samples was semi-quantitatively determined by using a 0-3+ scale, with 0 indicating no staining, 1+ indicating <10% staining, and 2+ indicating 10-50% staining, and 3+ indicating >50% staining. ANT samples [(A, B), top panels] were scored as 0, the Mohs cSCC samples [(A), bottom panel; arrows in the 400x image denote positive nuclear localization] were scored as 1+, and the advanced cSCC samples [(B), bottom panel; arrows in the 400x image denote positive nuclear localization] were scored as 2+. The boxes within the 40x and 100x images delineate the tissue location shown in the 100x and 400x images, respectively.