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. 2022 Jan 23;10(2):239. doi: 10.3390/biomedicines10020239

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Induced pluripotent stem cell (iPSC)-derived monocytes and macrophages (M_Φ). iPSC-derived monocytes were tested for the expression of monocyte markers, such as CD14, CD11b (A), and CD45 (B). iPSC derived monocytes were differentiated into macrophages in X-VIVO15 medium supplemented with M-CSF (100 ng/mL) for 7 days. Gating strategy for iPSC derived-M_Φ (iPSDM) is shown (C,D). iPSDM were stained for the macrophage marker CD68 (E) and tested for their phagocytic activity using fluorescein conjugated Zymosan particles (F).