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Validation of expression of novel polarisation markers in monocyte-derived macrophages (MDM). Peripheral blood monocyte-derived monocytes were differentiated to MΦ for 7 days and polarised with IFNγ + LPS or IL-4 for 48 h. Polarised MDM were stained for LILRB2, PILRA, CD300a, CD109, and Siglec-10 and analysed by FACS Canto. Representative of four independent donors.
