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HPLC-LTQ-MS analysis for 2-N’-acyl substituted KSM analogs generated using KasH. (A) EIC spectra of (i) KSM at m/z 380.13 [M+H], (ii) Ac-KSM at m/z 422.19 [M+H], (iii) isovaleryl-KSM at m/z 464.10 [M+H], (iv) β-hydroxybutyryl-KSM at m/z 466.24 [M+H], (v) acetoacetyl-KSM at m/z 464.35 [M+H], (vi) propionyl-KSM at m/z 436.05 [M+H]. (B) Chemical structures of 2-N’-acyl substituted KSM analogs with calculated m/z [M+H].
