Table 4.

Thermodynamic parameters of KasQ versus different substrates.

S.No	KasQ with	n	Kd (µM)	∆G (kcal mol−1)	∆H (kcal mol−1)	∆S (cal mol−1 K−1)
1	AMP	NBD	NBD	NBD	NBD	NBD
2	ADP	NBD	NBD	NBD	NBD	NBD
3	ATP	NBD	NBD	NBD	NBD	NBD
4	GMP	NBD	NBD	NBD	NBD	NBD
5	GDP	NBD	NBD	NBD	NBD	NBD
6	GTP	NBD	NBD	NBD	NBD	NBD
7	TTP	1.1 ± 0.1	174.5 ± 20.2	−5.128 ± 1.221	−7.612 ± 1.221	−8.33
8	UMP	1.0 ± 0.0	123.9 ± 70.9	−13.19 ± 1.245	−9.254 ± 1.245	13.2
9	UDP 3	1.0 ± 0.0	32.2 ± 6.8	−6.128 ± 0.5523	−4.130 ± 0.5523	6.70
10	UTP	1.0 ± 0.0	71.4 ± 5.7	−5.645 ± 1.340	−9.104 ± 1.340	−11.6
11	UDP-GlcNAc 1	NBD	NBD	NBD	NBD	NBD
12	UDP-GlcNAc/Mg2+	NBD	NBD	NBD	NBD	NBD
13	UDP-GalNAc	1.8 ± 0.4	334.4 ± 133. 7	−4.742 ± 1.700	−3.418 ± 1.700	4.44
14	UDP-Glc	0.5 ± 0.0	9.5 ± 0.3	−6.834 ± 0.0375	−0.3944 ± 0.0375	21.6
15	TDP-Glc	0.3 ± 0.0	25.1 ± 0.2	−6.273 ± 0.2773	−4.568 ± 0.2773	5.72
16	GDP-Glc	NBD	NBD	NBD	NBD	NBD
17	UDP-Gal	1.0 ± 0.2	273.3 ± 54.6	−4.862 ± 1.926	−6.636 ± 1.926	−5.95
18	UDP-GlcA	1.5 ± 0.2	76.3 ± 15.2	−5.61 ± 0.5751	−1.376 ± 0.5751	14.2
19	GDP-Man	NBD	NBD	NBD	NBD	NBD
20	UDP-GlcNH2	NBD	NBD	NBD	NBD	NBD
21	GlcNAc	NBD	NBD	NBD	NBD	NBD
22	GalNAc	NBD	NBD	NBD	NBD	NBD
23	GlcNH2	NBD	NBD	NBD	NBD	NBD
24	GlcA	NBD	NBD	NBD	NBD	NBD
25	GalA	NBD	NBD	NBD	NBD	NBD
26	D-Glucose	NBD	NBD	NBD	NBD	NBD
27	D-Mannose	NBD	NBD	NBD	NBD	NBD
28	D-Galactose	NBD	NBD	NBD	NBD	NBD
29	AAG 2	NBD	NBD	NBD	NBD	NBD

NBD: No binding detected.