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. 2022 Jan 28;11(2):213. doi: 10.3390/biology11020213

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Functional annotation of DEmRNAs with steroid hormone biosynthesis pathway in P. olivaceus. (a) GO and KEGG enrichment of testis- and ovary-biased DEmRNAs; (b) differential expression of selected genes in ovarian steroidogenesis pathway by RNA-seq and qRT-PCR; (c) DEmRNAs in the steroidogenesis pathway. Blue boxes represent testis-biased genes, red boxes represent ovary-biased genes, and yellow boxes represent genes expressed in both testis and ovary. Data are shown as mean ± SD (n = 3). Asterisks indicate statistical significance between groups (* p < 0.05; ** p < 0.01; *** p < 0.001).