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. 2022 Jan 28;11(2):213. doi: 10.3390/biology11020213

Figure 6.

Figure 6

Functional investigation of let-7/miR-125b cluster in gynogenetic P. olivaceus. Expression of six putative lncRNAs as let-7 precursors by (a) RNA-seq and (b) qRT-PCR; (c) expression of let-7, miR-125b and miR-100 by qRT-PCR; dual-luciferase reporter analysis of let-7 targeting cyp11a (d) and miR-125b targeting hsd3b1 (e) and esr2b (f), with the bold yellow regions as the complementary target sites. Data are shown as mean ± SD (n = 3). Asterisks indicate statistical significance between groups (* p < 0.05; ** p < 0.01; *** p < 0.001).