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. 2022 Feb 18;14(4):1043. doi: 10.3390/cancers14041043

Figure 4.

Figure 4

The kinetics of (−)-P-, (+)-P- and (R)-P-induced caspase 3/7 activation in HT29 cells grown as spheroids. HT29 spheroids were grown for 96 h, followed by live-cell imaging for a further 96 h in the presence of (−)-P, (+)-P and (R)-P and Caspase-3/7 Green reagent using an Incucyte S3 Live analysis system. (a) Representative images of HT29 spheroids 96 h post drug treatment. White bars represent 800 µm. (b) Caspase 3/7 kinetics curves generated by measuring the caspase 3/7 positive area within HT29 spheroids for (−)-P, (+)-P and (R)-P over 96 h. (c) Comparison of caspase 3/7 activation over 48 h after treatment with vehicle or 10 µM (−)-P, (+)-P and (R)-P. Individual points represent the mean ± SD from three technical replicates in a single experiment. *** p ≤ 0.001, **** p ≤ 0.0001. Two-way ANOVA with multiple comparisons test. Data are representative of two independent experiments.