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. Author manuscript; available in PMC: 2023 Feb 15.
Published in final edited form as: Circulation. 2022 Feb 14;145(7):531–548. doi: 10.1161/CIRCULATIONAHA.121.057301

Figure 4: Collagen expression in RHVD proteomes and identification of mimic epitopes with S. pyogenes “collagen-like surface protein”.

Figure 4:

A, Types of collagens found in human heart valve proteomes and collagen-like surface protein found in S. pyogenes (serotype M1) proteome available in UniProt database. Statistical differences (q<0.1) between abundances of human collagen in non-diseased valves and RHVD valves (NDAV versus RAVD and NDMV versus RMVD) are shown. B, Graphs display the collagen average abundances between NDAV and RAVD and between NDMV and RMVD. Type 1 collagens (COL1A1 and COL1A2) are highlighted in blue. Significantly differentially enriched proteins were calculated using a two-group comparison by adjusting individual p values from every pairwise comparison for significance (q<0.1). C, Homologue epitope regions between human COL1A1 and COL1A2 and S. pyogenes “collagen-like surface protein” found in as derived by multiple sequence alignment. Identical amino acids are marked with an asterisk, highly conserved positions with a colon, and homologue positions with a period. Sequences of nine amino acids (9-mer) with higher ability of binding to human leukocyte antigen class I (HLA-I) are highlighted in grey. “Class I immunogenicity score” and “percentile rank score” are defined by the peptide ability to elicit immune response and capacity of properly bind in the HLA-I respectively.