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. Author manuscript; available in PMC: 2023 Feb 15.
Published in final edited form as: Circulation. 2022 Feb 14;145(7):531–548. doi: 10.1161/CIRCULATIONAHA.121.057301

Figure 5: Peptide docking of type 1 collagen mimic epitopes and effects of recombinant ProTα on HLA-I expression by VICs.

Figure 5:

A, Peptide docking of four identified human collagen type 1 mimic epitopes (green) and homologous region on S. pyogenes (magenta) sequences showing their interaction on HLA-I groove. Peptides are positioned between α1 and α2 HLA-I domains. Binding energy scores are shown for each comparison pair. B, mRNA expression levels of HLA-A, B and by VICs non-stimulated (CTRL, white dots, n=3) and stimulated with recombinant ProTα for 24 hours (green, n=3). Bar graphs show the mean gene expression relative to the CTRL culture with error bars representing standard deviation. Two-group comparisons between non-stimulated and stimulated cell cultures were made using a paired t-test. Statistical significance is indicated in each graph.