Figure 7.

Asymptomatic hyperuricemia reduces interstitial fibrosis in acute organ injury. Hyperuricemic Alb-creERT2;Glut9^lox/lox (HU) and Glut9^lox/lox control (non-HU) mice were injected with tamoxifen and placed on a chow diet enriched with inosine to induce hyperuricemia (HU). Nephrectomy was performed (day 7) prior to clamping of the left renal pedicle for 15 min to induce ischemia/reperfusion injury (IRI). Mice were sacrificed on day 3 or 14. (A) Interstitial fibrosis illustrated on Picro-sirius red-stained kidney sections in non-HU and HU mice 3 and 14 days after IRI. Magnification, ×100. (B) Intrarenal mRNA expression levels of the fibrosis markers Col1α1 and fibronectin 1 in both groups of mice determined by RT-qPCR. Data are mean ± SEM from 7 mice per group. * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant; two-way ANOVA with Tukey’s post hoc test.