Figure 4.

Assessment of the oligodendroglial NPC-differentiation rate by quantification of Olig2⁺/GFP⁺ cells. (A) Immunofluorescence staining for Olig2 (blue), GFP⁺ (green) NPCs and Olig2⁺/GFP⁺ (B) The rate of oligodendroglial NPC-differentiation (Olig2⁺/GFP⁺ cells per GFP⁺ cells) is not significantly affected by stimulation or blockage of the Shh-pathway under normal and inflammatory growing conditions. Experiments performed in triplicate (n = 3), data presented as mean ± SEM, one-way-ANOVAs with post hoc Tukey-HSD tests performed for statistical analysis.