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Immuno-detection of the DREADD. (A) Immuno-fluorescent labeling of the HM4Di-CFP DREADD, the LC is clearly visible, just lateral to the fourth ventricle (IV), in spite of the green background covering the entire section. (B) A neighboring section was treated with immuno-histochemistry to reveal the expression of the HM4Di-CFP DREADD. The LC is also clearly visible, with little staining in surrounding nuclei.
