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. 2022 Feb 20;14(4):1071. doi: 10.3390/cancers14041071

Figure 2.

Figure 2

Lower levels of ADCK2 led to lower cell viability. (A): The melanoma cell line SkMel28 was transfected with siADCK2, and the cell viability was quantified for several time points after the transfection. The relative mRNA expression level of ADCK2 was reduced as early as 24 h post-transfection and even 96 h after transfection. The mRNA expression was normalized to SkMel28 siControl-transfected cells (n = 4). (B): Confirmation of a successful knockdown of ADCK2 96 h after transfection via Western blot. (C): SkMel28 cells were infected with either an empty vector (EV) control or an ADCK2 overexpression (OE) vector. The relative mRNA expression level increased in the ADCK2 OE cells about 3-fold compared with the EV-transfected cells (n = 4). (D): Confirmation of the overexpression of ADCK2 in OE ADCK2 cells via Western blot. (E): Lower levels of ADCK2 led to a significantly decreased cell viability of SkMel28 cells by about 20% after 24, 48, 72 and 96 h post-transfection compared to cells transfected with siControl (n = 6). (F): The overexpression of ADCK2 in SkMel28 cells led to a slightly increased cell viability compared to EV SkMel28 cells (time point 24 h) (n = 5). All statistical analyses were conducted with paired t-tests. * p ≤ 0.05; ** p ≤ 0.01; ns = not significant; RFU = relative fluorescent units.