Figure 2.

Effect of Dasatinib on melting curves, amplification plotsand histograms of gene expression profiles of SREBP, iNOS, TGF-β, PDGFα and GAPDH genes as studied by qRT-PCR in the model of obeosogenic diet-induced renal damage in mice. (A–E): Melting curves of the studied genes showing single sharp peaks confirming primers specificity. (F–J): Amplification plots “Linear view” showing the threshold which was set above the baseline in the exponential portion of the plot to accurately determine the threshold cycle (C_t) of the studied genes. (K–N): Histograms of the studied genes expression showing the means ± standard errors (SE) of genes fold change. Data are mentioned as mean ± SE, different letters = significant difference. SREBP1: Sterol regulatory element binding protein; iNOS: Inducible nitric oxide synthase; TGF-β: Transforming growth factor beta; PDGFA: Platelet-derived growth factor alpha.