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. 2022 Feb 9;11(4):590. doi: 10.3390/cells11040590

Figure 1.

Figure 1

DSS-induced colonic injury and inflammation in WT and IEC AMPK KO mice. (A) Experimental timeline for 4% DSS-induced colitis in female WT and IEC AMPK KO mice. Mice were treated with 4% DSS in drinking water for four consecutive days. Changes in (B) body weight and (C) colon length in WT and IEC AMPK KO mice on day 4 of 4% DSS-induced colitis (n = 5–10 mice/group). (D) In vivo paracellular intestinal epithelial permeability of WT and IEC AMPK KO mice on day 4 during 4% DSS-induced colitis protocol. Dextran flux was determined by measuring the amount of 4 kDa TRITC-dextran in the plasma 4 h after gavage (n = 10–14 mice/group). (E) Expression of mRNA for Il-6, TNF-α and IL-1β in the colon from WT and IEC AMPK KO mice on day 4 (n = 16–23 mice/group). The results are pooled from four independent experiments. Data are expressed as means ± SD. Statistical analysis was performed by one-way ANOVA with the post hoc Bonferroni’s multiple comparisons test; * p < 0.05, ** p < 0.01 and *** p < 0.001 indicate a significant change relative to the untreated condition.