Figure 2.

Concomitant loss of CtIP and BARD1 triggers apoptosis by DNA damage. (A,B) U2OS cells transfected with the indicated siRNA for 48 h were harvested and induction of apoptosis was determined by annexin V (A) or cleaved caspase-3 (B). Statistical significance was calculated with unpaired t-test. * p value ≤ 0.05, ns p value > 0.05. (C) Same cells as in (A) were fixed, permeabilized and immunostained with anti-γH2AX antibody along with DAPI before analysis by flow cytometry. Quantification gates were established in samples transfected with siCNTL. Percentages indicate mean ± s.e.m. of γH2AX-positive cells (n 3). Statistical significance was calculated with unpaired t-test. * p value ≤ 0.05, ** p value ≤ 0.01.