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Optimization of p53-cAb-DNA (2.5, 5, 10, and 25 fmol/mL) and anti-human-IgG-FB (0.015, 0.035, 0.07, 0.175, and 0.35 fmol/mL) concentrations for PIC detection. The PIC (pg/mL) levels were (a) 5000, (b) 2500, (c) 1250, (d) 625, (e) 312; (f) linearity in the serial dilution of 5000 pg/mL of PIC using 0.07 fmol/mL of anti-human-IgG-FB and 10 fmol/mL of p53-cAb-DNA.
