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. 2022 Feb 16;11(4):700. doi: 10.3390/cells11040700

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Significantly increased antigen-specific antibody levels in Cd19^Cre^/+mice following immunization with NP-Ficoll. Mice (female, ~10 weeks of age) were immunized with NP-Ficoll (10 μg/100 μL/mouse in PBS) intraperitoneally on D0. Levels of NP-specific IgM, IgG1 and IgG3 in sera of Cd19^Cre/+ or control WT littermates on D7 (A), D14/D21 (B), and D35 (C) post-immunization were determined by ELISA. Each symbol represents a single mouse of the indicated genotype, and results are expressed as mean ± SEM. * p < 0.05; ** p < 0.01.