Table 2.
Cox proportional hazards modelling
| Blood (n = 248) | CSF (n = 102) | |||||
|---|---|---|---|---|---|---|
| HR [95% confidence interval] | P | Adj P | HR [95% confidence interval] | P | Adj P | |
| Age of onset | 1.39 [0.96–2.02] | 0.087 | 0.347 | 1.04 [0.63–1.71] | 0.880 | 0.880 |
| FVC | 0.62 [0.40–0.98] | 0.051 | 0.308 | 0.77 [0.44–1.35] | 0.382 | 0.880 |
| Spinal onset | 1.39 [0.96–2.02] | 0.087 | 0.347 | 1.04 [0.63–1.71] | 0.880 | 0.880 |
| PR | 0.77 [0.38–1.57] | 0.483 | 0.828 | 1.43 [0.94–2.17] | 0.117 | 0.528 |
| ALS-specific ECAS score | 1.16 [0.71–1.89] | 0.572 | 0.858 | 1.17 [0.57–2.41] | 0.680 | 0.880 |
| Latency from symptom onset | 0.74 [0.50–1.11] | 0.159 | 0.477 | 0.87 [0.49–1.54] | 0.643 | 0.880 |
| Plasma NFL | 2.99 [1.65–5.41] | 0.001 | 0.016 | 5.13 [1.05–25.19] | 0.062 | 0.528 |
| CSF NFL | – | – | – | 1.12 [0.30–4.16] | 0.871 | 0.880 |
| CSF CHIT1 | – | – | – | 0.91 [0.34–2.41] | 0.853 | 0.880 |
| C3 | 1.38 [0.70–2.69] | 0.367 | 0.735 | – | – | – |
| C4 | 0.97 [0.50–1.88] | 0.918 | 0.918 | – | – | – |
| CRP | 0.96 [0.54–1.68] | 0.876 | 0.918 | – | – | – |
| Ferritin | 0.91 [0.51–1.61] | 0.741 | 0.918 | – | – | – |
| CK | 0.78 [0.50–1.20] | 0.268 | 0.644 | – | – | – |
Imputed datasets, incorporating available clinical parameters with all blood biochemical analytes or CSF analytes with plasma NFL. These indicate a significant independent association for plasma NFL in models of blood analytes, but no significant associations for CSF analytes with plasma NFL. Bold values indicate adjusted P-value <0.05.
HR, hazard ratio; CI, confidence interval; FVC, forced vital capacity; PR, progression rate; ALS, amyotrophic lateral sclerosis; ECAS, Edinburgh Cognitive and Behavioural ALS Screen; NFL, neurofilament light chain; CHIT1, chitotriosidase 1; C3, complement C3 protein; C4, complement C4 protein; CRP, C-reactive protein; CK, creatine kinase.