Figure 5.

In vitro uptake of the different Pba formulations in MDA-MB-231 (a), MCF7 (b) and 4T1 (c) cells cultured as monolayers. Cells were incubated for 1 or 4h with 0.25 μM of Pba dissolved in the standard solvent or loaded in MAL-PTX₂S@Pba or in BOC-PTX₂S@Pba, and the uptake of Pba was measured by flow cytometry at the end of incubation times. Data are expressed as mean percentage ± SD of least three independent experiments, carried out in triplicate. Statistical significance was calculated applying the two-way ANOVA with Bonferroni’s correction: ● significantly different from BOC-PTX₂S@Pba; # significantly different from Pba.