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. 2022 Feb 14;11(4):660. doi: 10.3390/cells11040660

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Treatment with PF-04957325 suppresses the accumulation of IL-10-producing CD4⁺ T cells in the spinal cord. Mice with EAE scores of at least 1 were treated with the vehicle control or PF-04957325 thrice daily, for 6 to 13 days. (A) The detection of IL-10⁺ CD4⁺ cells after ex vivo restimulation with PMA/Ionomycin by flow cytometry in the spinal cord mononuclear cells (CNS) of EAE mice treated with the vehicle control or PF-04957325. The representative dot plots show that CD4⁺ T cells (x-axis) that are positive for IL-10⁺ (y-axis) show comparable frequencies in the vehicle- and inhibitor-treated mice (** p = 0.002). (B) The data represent the total number of IL-10⁺ CD4⁺ T cells (n = eight mice per group, mean ± SEM of three independent experiments).

Treatment with PF-04957325 suppresses the accumulation of IL-10-producing CD4⁺ T cells in the spinal cord. Mice with EAE scores of at least 1 were treated with the vehicle control or PF-04957325 thrice daily, for 6 to 13 days. (A) The detection of IL-10⁺ CD4⁺ cells after ex vivo restimulation with PMA/Ionomycin by flow cytometry in the spinal cord mononuclear cells (CNS) of EAE mice treated with the vehicle control or PF-04957325. The representative dot plots show that CD4⁺ T cells (x-axis) that are positive for IL-10⁺ (y-axis) show comparable frequencies in the vehicle- and inhibitor-treated mice (** p = 0.002). (B) The data represent the total number of IL-10⁺ CD4⁺ T cells (n = eight mice per group, mean ± SEM of three independent experiments).