Table 1.
RT-PCR primers used in reverse transcription.
| Target Genes | GenBank Accession | Primer Sequence | |
|---|---|---|---|
| PPAR-γ | NM_001308354.1 | Forward | 5-GAA AGA CAA CGG ACA AAT CAC-3 |
| Reverse | 5-GAA ACT GGC ACC CTT GAA-3 | ||
| HMGCR | NM_001360165.1 | Forward | 5-AGA ATA ATG TGC TAA GTA GTG CTA A-3 |
| Reverse | 5-GCC TCT CTG AAC AAA GAC TC-3 | ||
| SREBP-1C | NM_001358315.1 | Forward | 5-CTT CTG GAG ACA TCG CAA AC-3 |
| Reverse | 5-GGT AGA CAA CAG CCG CAT C-3 | ||
| FAS | NM_007988.3 | Forward | 5-CTT GGG TGC TGA CTA CAA CC-3 |
| Reverse | 5-GCC CTC CCG TAC ACT CAC TC-3 | ||
| HSL | NM_010719.5 | Forward | 5-AAG GAC TCA CCG CTG ACT TCC-3 |
| Reverse | 5-GCC TGT CTC GTT GCG TTT GTA-3 | ||
| ATGL | NM_025802.3 | Forward | 5-GAC CTG ATG ACC ACC CTT TCC-3 |
| Reverse | 5-TGC TAC CCG TCT GCT CTT TCA-3 | ||
| DGAT | NM_010046.3 | Forward | 5-CCT CAG CCT TCT TCC ATG AG-3 |
| Reverse | 5-ACT GGG GCA TCG TAG TTG AG-3 | ||
| GAPDH | NM_001289726.1 | Forward | 5-GCA CAG TCA AGG CCG AGA AT-3 |
| Reverse | 5-GCC TTC TCC ATG GTG GTG AA-3 | ||
Peroxisome proliferator-activated receptors (PPAR-γ), 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR), sterol regulatory element-binding protein 1 (SREBP-1C), fatty acid synthase (FAS), hormone-sensitive triglyceride lipase (HSL), adipose triglyceride lipase (ATGL), Diacylglycerolacyl transferase (DGAT), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH).