Video 1. TMEM16 A is required for the generation of spontaneous volume changes in Kölliker’s organ (KÖ).

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Time-lapse imaging (one image per second) reveals spontaneous volume changes of inner supporting cells of in a wildtype mouse cochlea (P7), which propagate in a wave-like manner up and down the cochlea turn. In contrast, volume changes are almost absent in the cochlea isolated from a cKO littermate (the bottom of the video shows erythrocytes moving in a blood vessel). Images were processed using a custom-written ImageJ macro and the ImageJ software. Each frame was subtracted from an average of five preceding frames to highlight the changes in light scattering caused by the changes in cell volume. The video (seven images per second) shows the top view of an area from an isolated cochlea turn. KÖ, Kölliker's organ; IHC, inner hair cells.