Video 2. TMEM16A is required for the propagation of cochlear Ca²⁺ waves.

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Time-lapse imaging (one image per second) reveals spontaneous Ca²⁺ signals in the inner supporting cells that propagate up and down the cochlear turn in a wildtype mouse cochlea (P7). In contrast, Ca²⁺ waves are reduced to local Ca²⁺ events in the cochlea of a cKO littermate. Images were processed using a custom-written ImageJ macro and the ImageJ software. Each frame was subtracted from an average of five preceding frames to highlight the changes in Ca²⁺ concentration. The video (seven images per second) shows the top view of an area from an isolated cochlea turn. KÖ, Kölliker’s organ; IHC, inner hair cells.