TABLE 2.
Drug resistance mutations observed in HIV protease and reverse transcriptase
| Sample | Extraction resulta | No. of HIV RNA copies/mlb | Mutation at indicated positionc in:
|
||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Protease
|
Reverse transcriptase
|
||||||||||||||||||||
| 10 (L/I) | 36 (M) | 46 (M) | 63 (L/T) | 64 (I) | 71 (A) | 77 (V) | 82 (V) | 90 (L) | 41 (M) | 67 (D) | 69 (T) | 70 (K) | 74 (L) | 103 (K) | 184 (M) | 214 (F) | 215 (T) | 219 (K) | |||
| 93 | N+ and Q− | 399 | V | − | − | − | − | − | − | − | − | − | N | D | R | − | − | − | L | V | Q |
| 151 | N and Q+ | 666 | − | L | − | − | V | − | − | − | − | − | N | − | R | − | N | V | − | Y | Q |
| 195 | N+ and Q− | 1,083 | − | − | − | P | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − |
| 369 | Q+ and N− | 385 | − | − | − | − | − | T | I | − | − | − | N | − | R | − | − | V | − | − | − |
| 455 | N+ and Q− | 154 | − | − | I | P | − | T | I | T | M | L | − | − | − | V | − | V | − | Y | − |
a
RNA extraction method; N, NucliSens; Q, QIAamp.
b
bDNA version 3.0.
c
Consensus HIV amino acids of HIV strains MN and HXB2 are shown in parentheses. Dashes indicate homology with the consensus sequence.