Table 4.

Expression stability ranking of the candidate references in Japanese flounder livers with Ringer’s solution or E. tarda injection at different time points. The best performers, miR-22-3p and miR-23a-3p, are shown in bold.

Method	Ranking Order (Better--Good--Average)
Delta CT	miR-210-5p	miR-22-3p	miR-23a-3p	miR-205-5p	miR-30c-5p	18S rRNA	miR-34a-5p	U6	miR-101a-3p	5S rRNA
BestKeeper	5S rRNA	miR-101a-3p	miR-34a-5p	miR-30c-5p	miR-210-5p	18S rRNA	miR-205-5p	miR-22-3p	miR-23a-3p	U6
NormFinder	miR-210-5p	miR-22-3p	miR-23a-3p	miR-205-5p	miR-30c-5p	18S rRNA	miR-34a-5p	U6	miR-101a-3p	5S rRNA
geNorm	miR-22-3p|miR-23a-3p		miR-210-5p	18S rRNA	miR-205-5p	miR-30c-5p	U6	miR-34a-5p	miR-101a-3p	5S rRNA
Recommended comprehensive ranking	miR-210-5p	miR-22-3p	miR-23a-3p	miR-205-5p	miR-30c-5p	18S rRNA	miR-34a-5p	5S rRNA	miR-101a-3p	U6