Figure 7.

RNA interference in primary HUVEC. STAT3 silencing was performed by siRNA transfection using an RNAiMAX kit for 48 h before the primary HUVEC were lysed for Western blot (A–C) or seeded on the gelled Matrigel and treated with 20 μM of ASI for 6 h for tube formation assay (D–E). A. Representative photographs of Western blot. B. Statistical analysis of STAT3 and p-STAT3. C. Statistical analysis of VEGF. D. Representative photographs of tube formation assay. E. Statistical analysis of tube length. All experiments were repeated at least three times. Data are presented as Mean ± SD. N = 6. *p < 0.05, **p < 0.01. NS, no significance.