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. 2019 Oct 17;57(1):717–728. doi: 10.1080/13880209.2019.1672755

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Electrophoretic separation of HA and its derivatives. The HA ladder (standard) has a range of molecular weight of HA from 500 to 30 kDa. The amounts of analytes that were loaded on the gel are as follows: 7.5 μg of native HA; 7.5 μg of DHA; 5 μg of DHA1, prepared via the same method as to prepare DHA but from a different batch; 7.5 μg of AHA, partially deacetylated HA and then reacylated with acetic anhydride; 7.5 µg of BHA; 7.5 μg of BHA1, prepared via the same method as to prepare BHA, but from a different batch.