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. 2022 Feb 2;13(2):295. doi: 10.3390/genes13020295

Figure 5.

Figure 5

Expression and localization of Kiss1 in rat epididymis. (A) Western blot for Kiss1 in spermatozoa free epididymis caput and cauda. (B) Western blot for MSJ-1, used as a specific marker of spermatozoa. (C) Schematic representation of cell types in epididymal epithelium, with Kiss1 immunoreactive cells in beige. 1: Myoid cells; 2: basal cells; 3: apical cells, 4: Halo cell, 5: principal cells, 6: clear cells, L: lumen, SPZ: spermatozoa. (D–H) Histological sections of caput epididymis; (I–M) Histological sections of cauda epididymis. (D,I): Haematoxylin-eosin staining. (E–G,J–L): Immunohistochemistry analysis for Kiss1. (H,M): controls of immunoreactions. Arrows indicate Kiss1 immunoreactive cells. Asterisk indicates statistically significant differences (p < 0.05).