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. 2022 Feb 2;13(2):295. doi: 10.3390/genes13020295

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression and localization of Kiss1R in rat epididymis. (A) Western blot for Kiss1R in spermatozoa free epididymis (Ep.) caput and cauda. (B) Schematic representation of cell types in epididymal epithelium, with Kiss1R immunoreactive cells in violet. 1: Myoid cells; 2: basal cells; 3: apical cells, 4: Halo cell, 5: principal cells, 6: clear cells, L: lumen, SPZ: spermatozoa. (C–G) Histological sections of caput epididymis; (H–L) Histological sections of causa epididymis. (C,H) Haematoxylin-eosin staining. (D–G), (I–L): immunohistochemistry analysis for Kiss1R. (G,L): controls of immunoreactions. Arrows indicate Kiss1R immunoreactive cells. Asterisk indicates statistically significant differences (p < 0.05).