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. 2022 Jan 27;13(2):241. doi: 10.3390/genes13020241

Figure 5.

Figure 5

Effect of HB and AB microglia-conditioned media on ACM-induced ALS phenotype. (A) Representative image of HB motor neurons (MNs) treated with medium from AB astrocytes pre-treated with HB microglia-conditioned media (HB MCM) (red = NF200, green = phNF, blue = DAPI 63× objective, scale bars = 10 μm). (B) Quantification confirms no change in NF subunit expression in HB MNs following treatment with AB ACM pre-treated with HB MCM [t-test, ns = 0.7795]. (C) Quantification shows prevention of ACM induced phNF+ aggregates in HB MNs after pre-treatment of AB astrocytes with HB MCM [t-test, ns = 0.4340]. (D) Representative image of AB MNs treated with AB ACM pre-treated with HB MCM (red = NF200, green = phNF, blue = DAPI 63× objective). (E) Quantification confirms no change in NF subunit expression in AB MNs after application of AB ACM pre-treated with HB MCM [t-test, ns = 0.3222]. (F) Quantification reveals a significant reduction in phNF+ aggregates when HB MCM pre-treated AB ACM is applied to AB MNs [t-test, * = 0.0203].