TABLE 2.

Summary of optimal fungal inoculum dilutions for RSA and comparison of the MIC_RSAs to published MICs

Antifungal agent	Isolate	RSA incubation time (h)	Optimal inoculum dilution range for the RSAa	MICRSA range (μg/ml) for optimal inoculum dilutions	Published (μg/ml)b MIC range
Amphotericin B	C. albicans ATCC 24433	6	1/8	0.25	0.25–1.0
		19	1/512–1/8192	0.125–0.25
	C. albicans ATCC 90028	6	1/8	0.5	0.5–2.0
		19	1/8–1/8192	0.5
	C. tropicalis ATCC 750	6	1/8	0.5	0.5–2.0
		19	1/8–1/8192	0.5
	C. krusei ATCC 6258	6	1/8	1.0	0.5–2.0
		19	1/8–1/2048	0.5–1.0
	C. parapsilosis ATCC 22019	6	1/8	1.0	0.25–1.0
		19	1/2–1/128	0.5–1.0
	C. parapsilosis ATCC 90018	6	1/2–1/8	0.5–1.0	0.5–2.0
		19	1/2–1/128	0.5–1.0
Fluconazole	C. albicans ATCC 24433	6	NDc		0.25–1.0
		19	1/512–1/8192	0.5
	C. albicans ATCC 90028	6	ND		0.25–1.0
		19	1/512–1/8192	0.5–1.0
	C. tropicalis ATCC 750	6	1/8	1.0	1.0–4.0
		19	1/512–1/8192	1.0–2.0
	C. krusei ATCC 6258	6	ND		16.0–64.0
		19	1/512–1/2048	32–64
	C. parapsilosis ATCC 22019	6	ND		2.0–8.0
		19	1/8–1/128	2.0–4.0
	C. parapsilosis ATCC 90018	6	ND		0.25–1.0
		19	1/8–1/128	1.0

^aThe greatest inoculum dilution range across which the endpoint was stable within two consecutive drug concentrations. 

^bObtained from reference 11. 

^cND, no dilution evaluated filled the criteria; thus, a determination of an optimal inoculum for the RSA could not be made.