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. 2022 Jan 18;13(2):166. doi: 10.3390/genes13020166

Table 1.

Oligonucleotides used in this study.

Sequence, 5′→3′ Length Function
GCTCTGGAATTCCTTCXCTTCTTTCCTCTCGACGGTCTCG
X = 8-oxoguanine
40 Template for ss-DPC or temp-DPC, cross-link at X
GCTCTGGAATTCCTTCGCTTCTTTCCTCTCGACGGTCTCG 40 Template for undamaged substrates
GCTCTGGAATTCCTTCCCTTCTTTCCTCTCGACGGTCTCG 40 Template for down-DPC
GAGGAAAGAAGXGAAGGAATTCCAGAGC
X = 8-oxoguanine
28 Displaced strand for down-DPC, cross-link at X
GAGGAAAGAAGCGAAGGAATTCCAGAGC 28 Displaced strand for undamaged substrates
CGAGACCGTCG 11 Primer and size marker M1
CGAGACCGTCGCGAGGAAAGAAG 23 Size marker M2, corresponding to primer elongation to the cross-link site
CGAGACCGTCGCGAGGAAAGAAGCGAAGGAATTCCAGAGC 40 Size marker M3, corresponding to the full-size product