Table 1.
Sequence, 5′→3′ | Length | Function |
---|---|---|
GCTCTGGAATTCCTTCXCTTCTTTCCTCTCGACGGTCTCG X = 8-oxoguanine |
40 | Template for ss-DPC or temp-DPC, cross-link at X |
GCTCTGGAATTCCTTCGCTTCTTTCCTCTCGACGGTCTCG | 40 | Template for undamaged substrates |
GCTCTGGAATTCCTTCCCTTCTTTCCTCTCGACGGTCTCG | 40 | Template for down-DPC |
GAGGAAAGAAGXGAAGGAATTCCAGAGC X = 8-oxoguanine |
28 | Displaced strand for down-DPC, cross-link at X |
GAGGAAAGAAGCGAAGGAATTCCAGAGC | 28 | Displaced strand for undamaged substrates |
CGAGACCGTCG | 11 | Primer and size marker M1 |
CGAGACCGTCGCGAGGAAAGAAG | 23 | Size marker M2, corresponding to primer elongation to the cross-link site |
CGAGACCGTCGCGAGGAAAGAAGCGAAGGAATTCCAGAGC | 40 | Size marker M3, corresponding to the full-size product |