TABLE 1.

Reaction buffers for the DNA polymerases

DNA polymerase	Reaction buffer components
AmpliTaq Gold	10 mM Tris-HCl (pH 8.3), 50 mM KCl, 2.5 mM MgCl2, and 0.75 U of AmpliTaq Gold DNA polymerase (Perkin-Elmer Cetus)
DyNAzyme II	10 mM Tris-HCl (pH 8.8; 25°C), 1.5 mM MgCl2, 50 mM KCl, 0.1% Triton X-100, and 1 U of DyNAzyme II DNA polymerase (FINNZYMES OY, Riihitontuntie, Espoo, Finland)
DyNAzyme EXL	50 mM Tris-HCl (pH 9.0; 25°C), 2.5 mM MgCl2, 15 mM (NH4)2SO4, 0.1% Triton X-100, and 1 U DyNAzyme EXT DNA polymerase (FINNZYMES OY)
Expand high fidelity	1× Expand H F buffer, 1.5 mM MgCl2, and 1.33 U of Expand H F PCR system (Roche Molecular Biochemicals, Basel, Switzerland)
HotTub	50 mM Tris-HCl (pH 9.0), 20 mM (NH4)2SO4, 2.5 mM MgCl2, and 0.75 U of HotTub DNA polymerase (Amersham Pharmacia Biotech)
Pwo	10 mM Tris-HCl (pH 8.85; 20°C), 25 mM KCl, 5 mM (NH4)2SO4, 1.5 mM MgCl2, and 1.25 U of Pwo DNA polymerase (Roche Molecular Biochemicals)
rTth	10 mM Tris-HCl (pH 8.3), 5% (vol/vol) glycerol, 0.1 M KCl, 0.05% (w/vol) Tween 20, 0.75 mM EGTA ([ethylene glycol-bis (β-aminoethyl ether)]-N, N, N′, N′-tetraacetic acid) 2.5 mM MgCl2, and 1.25 U of rTth DNA polymerase (Perkin-Elmer Cetus)
Taq	10 mM Tris-HCl, 1.5 mM MgCl2, 50 mM KCl (pH 8.3; 20°C), and 0.75 U of Taq DNA polymerase (Roche Molecular Biochemicals)
Tfl	20 mM Tris-acetate (pH 9.0), 10 mM (NH4)2SO4, 75 mM potassium acetate, 0.05% Tween 20, 2.5 mM MgSO4, and 0.5 U of Tfl DNA polymerase (Promega Corporation, Madison, Wis.)
Tli	10 mM Tris-HCl (pH 9.0; 25°C), 0.1% Triton X-100, 50 mM KCl, 2.5 mM MgCl2, and 0.3 U of Tli DNA polymerase (Promega Corporation)
Ultma	10 mM Tris-HCl (pH 8.8; room temperature), 10 mM KCl, 0.002% (vol/vol) Tween 20, 2.5 mM MgCl2, and 0.75 U of Ultma DNA polymerase (Perkin-Elmer Cetus)