TABLE 3.
Inhibitory effects of different size-exclusion chromatography fractions of plasma on PCRa
| Peak no. | Molecular massb (kDa) | PCR resultsc at dilution of:
|
||||
|---|---|---|---|---|---|---|
| Undiluted | 1:10 | 1:50 | 1:100 | 1:200 | ||
| P1 | ∼800 | − | − | + | + | + |
| P2 | ∼200 | − | − | − | − | + |
| P3 | ∼80 | − | − | + | + | + |
| P4 | <13 | + | + | + | + | + |
| P5 | <13 | + | + | + | + | + |
| P6 | <13 | + | + | + | + | + |
Ability of AmpliTaq Gold to amplify 1 ng of L. monocytogenes DNA in the presence of 5 μl of different dilutions of plasma fractions.
Peaks P4, P5, and P6 were outside the range of the size marker.
Symbols: +, band of high intensity; −, no band.